在電泳時,溫度不均勻會帶來一係列複雜後果。局部溫度過高會使緩衝液黏度降低,離子遷移速度加快,導致條帶變形、分fen辨bian率lv下xia降jiang。比bi如ru在zai蛋dan白bai質zhi電dian泳yong中zhong,高gao溫wen可ke能neng引yin起qi蛋dan白bai質zhi變bian性xing,改gai變bian其qi原yuan有you結jie構gou和he電dian荷he分fen布bu,使shi條tiao帶dai出chu現xian拖tuo尾wei或huo彌mi散san現xian象xiang。而er且qie不bu同tong區qu域yu溫wen度du差cha異yi會hui造zao成cheng電dian場chang分fen布bu不bu均jun,影ying響xiang分fen子zi的de正zheng常chang泳yong動dong軌gui跡ji。
此(ci)外(wai),溫(wen)度(du)不(bu)均(jun)勻(yun)還(hai)可(ke)能(neng)引(yin)發(fa)對(dui)流(liu),擾(rao)亂(luan)分(fen)子(zi)的(de)有(you)序(xu)遷(qian)移(yi),使(shi)得(de)結(jie)果(guo)難(nan)以(yi)準(zhun)確(que)分(fen)析(xi)。為(wei)避(bi)免(mian)這(zhe)些(xie)問(wen)題(ti),常(chang)需(xu)使(shi)用(yong)冷(leng)卻(que)裝(zhuang)置(zhi)來(lai)維(wei)持(chi)電(dian)泳(yong)過(guo)程(cheng)中(zhong)溫(wen)度(du)的(de)穩(wen)定(ding)。